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Nucleic Acid Encoding Fluorescent Protein Constructs and Methods for Detecting Apoptosis

總結

The advanced clothing functional design CAD simulation technologies provide a multi-style garment thermal functional design simulation which can estimate the best material and garment requirements needed for environment- / weathered-related situations. It increases efficiency and decreases production costs by saving time and money invested on sample making.

技術優勢

The software is also a useful tool for training institutions offering courses on design and product development. Students can make use of the interactive platform to understand better the influence of different factors such as weather, environment and biological elements on clothing design.

技術應用

- Laboratories in the world studying apoptosis and related diseases
- Research conducted in scientific institutes or in the pharmaceutical industry for high throughput anti-cancer drug screening

詳細技術說明

Database of materials, 3D human body models and garment pieces of different styles

附加資料

Patent Number: US7354735B2
Application Number: US2003341979A
Inventor: Chang, Donald Choy | Luo, Qian (Kathy)
Priority Date: 24 May 2001
Priority Number: US7354735B2
Application Date: 11 Jan 2003
Publication Date: 8 Apr 2008
IPC Current: C12P002106 | C12Q000137 | G01N0033542
US Class: 4350691 | 4241921 | 4350071 | 4350072 | 4350074 | 4352523 | 4353201 | 435325 | 530300 | 530350 | 5360231
Assignee Applicant: The Hong Kong University of Science & Technology
Title: Nucleic acid encoding fluorescent protein constructs and methods for detecting apoptosis
Usefulness: Nucleic acid encoding fluorescent protein constructs and methods for detecting apoptosis
Summary: (IV) containing (I) or (II) is useful for detecting caspase or protease activated apoptosis which involves culturing (IV) containing (I) or (II) under conditions suitable for excitation of the donor fluorescent protein and detecting a fluorescent property in the sample, where the presence of caspase activated apoptosis or programmed cell death in the cells of the sample results in a change in the degree of fluorescence resonance energy transfer between the donor protein and the acceptor protein. The method further involves quantification of the change in the degree of fluorescent energy transfer. (IV) containing (I) or (II) is useful for determining whether an agent modifies caspase or protease activated apoptosis in a host which involves contacting (IV) containing (I) or (II) with the agent and culturing the cells under conditions suitable for excitation of the donor fluorescent protein and determining a fluorescent property of the sample, where an activity of the agent is determined by a change in the degree of the fluorescent property in the presence and absence of the agent. The agent is chosen from small molecule compound, protein, nucleic acid, ribozyme, antisense nucleic acid and isolated compounds from traditional Chinese medicine and their chemical derivatives. The method further involves providing a second sample of host cells and culturing the second sample under conditions suitable for exciting the donor fluorescent protein and comparing the degree of fluorescence resonance energy transfer in two samples (all claimed).
Novelty: Fluorescent protein construct useful for detecting caspase or protease activated apoptosis, having donor and acceptor fluorescent protein linked by peptide linker with substrate sequence of caspase or protease