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Efficient microfluidic device for rapid development and screening of oligonucleotide aptamers

Summary

Aptamers are small oligonucleotides capable of recognizing and binding to molecules in complex biological environments, making them useful tools for basic research, clinical diagnostics, and therapy. Conventional techniques for developing new aptamers involve labor-intensive screening techniques that take months to complete, slowing their deployment and widespread use. This technology is a microfluidic device for the iterative screening and development of aptamers against a user-defined target. This device can produce high-affinity aptamers in a few hours at reaction volumes of less than five microliters. As such, this technology could greatly improve the diversity and quality of aptamers in biomedical research and advance personalized medicine through creating patient-specific diagnostic and therapeutic tools.

Technology Benefits

Extremely rapid aptamer developmentOperates with minimal manual intervention Uses operationally benign conditions and is compatible with a wide variety of sample typesPatent Information:Patent Pending (WO/2015/143442)Tech Ventures Reference: IR CU14218, IR CU15101, IR CU15165

Technology Application

Development of aptamers against protein targets for researchDevelopment of aptamers against patient-derived samples for clinical useDevelopment of aptamers that recognize specific small molecules for drug development and metabolomics workTool for personalized diagnostics and therapeutics

Detailed Technology Description

None

*Abstract

None

*Inquiry

Richard NguyenColumbia Technology VenturesTel: (212) 854-8444Email: TechTransfer@columbia.edu

*IR

CU15165

*Principal Investigator

*Publications

Kim, J., Olsen, T., Zhu, J., Hilton, J.P., Yang, K-A., Pei, R., Stojanovic, M.N., Lin, Q. “Integrated Microfluidic Isolation of Aptamers Using Electrophoretic Oligonucleotide Manipulation” Scientific Reports. 2016 May 24;6:26139.

Country/Region

USA